The Mass Spectrometry Core at Drexel University College of Medicine
Introduction
The Mass Spectrometry Core Facility here at Drexel University College of Medicine is a new facility started at the beginning of 2008 to facilitate the research of those within Drexel as well as those people outside the Drexel family who are in need of precise and accurate mass spectrometry work in their current and upcoming projects. Although small now, we believe that this facility will ultimately grow into a well-known and respected research core which reflects the excellence by which each and every lab at Drexel prides itself on.
Who are we?
Keith Vosseller, Ph.D., Director of Mass Spectrometric Research
John D. Deuso, Manager, Mass Spectrometry Core Facility
Jenny Sherwood, Biochemistry Department Administrator
Instrumentation
At the core of our facility is a Thermo Finnigan LTQ triple-quad mass spectrometer. On the front end is an Eksigent NanoLC-2D liquid chromatography system running very small sample volumes (<500 nL/minute) into the nanospray ionization (NSI) source.
Services
A price sheet can be found here for all of the services below.
Trypsin digest of Commasie-stained G250 gel bands
Upon receipt of gel band, we will destain the band, digest the band using sequencing grade Trypsin, extract the protein fragments from the sample, desalt using Millipore Zip-Tips and resuspend in a buffer of your choice.
Trypsin digest of in-solution protein mixtures
Upon receipt of protein mixture, we will digest the band using sequencing grade Trypsin, extract the protein fragments from the sample, desalt using Millipore Zip-Tips, dry down and resuspend in a buffer of your choice.
Reverse phase LC/MS/MS of trypsin digest
The sample will be dried, and resuspended (desalted if necessary) for running in the Thermo-Finnigan LTQ Mass Spectrometer. The sample will be run using information on what the search parameters should be from the customer, and raw data will be returned. For very complex mixtures, we will also run strong cation exchange (up to 15 fractions) and run each separately on the LTQ for better peptide sensitivity and results.
Data interpretation
We will take the data from the LTQ run and analyze it for you, finding answers to your most complex mass spectrometry related questions.
Static nanospray of small molecules or peptides
Upon receipt of purified sample, the sample will be injected to find accurate molecular weight of the molecule. Perfect for checking the results of your peptide or small molecule synthesis.
Other services, such as peptide quantification and purification using IMAC beads are also available. Please inquire to John Deuso at JDeuso@Drexelmed.edu regarding other services as well as for a comprehensive pricing sheet for our services.
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